![]() ![]() Prism can fit standard curves using nonlinear regression (curve fitting), linear regression, or a cubic spline (or LOWESS) curve. Step 6 is to insert the results of the interpolation in a table on the chart. ![]() Steps 3-5 are to format the graph to make it more informative. Step 2 is to fit a curve or line to the data and determine the concentrations of the unknown samples. The results are graphed with the known concentrations on the X axis and the measured variable on the Y axis. Step 1 is to construct a standard curve that defines the relationship between the known concentrations of a substance and a measured value such as optical density, fluorescent intensity, radioactivity, etc. Prism 6 Step-by-Step Example Linear Standard Curves Interpolating from a standard curve is a common way of quantifying the concentration of a sample.
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